期刊
EMBO REPORTS
卷 19, 期 12, 页码 -出版社
WILEY
DOI: 10.15252/embr.201846433
关键词
APC/C; Calcineurin; Cdc20; Meiosis; Xenopus laevis; XErp1
资金
- German Research Foundation (DFG) [CRC 969]
- Konstanz Research School Chemical Biology (KoRS-CB)
- MRC [MC_UP_1201/6] Funding Source: UKRI
Vertebrate oocytes await fertilization arrested at metaphase of the second meiotic division. Fertilization triggers a transient calcium wave, which induces the activation of the anaphase-promoting complex/cyclosome (APC/C) and its co-activator Cdc20 resulting in the destruction of cyclin B and hence meiotic exit. Two calcium-dependent enzymes are implicated in fertilization-induced APC/C-Cdc20 activation: calcium-/calmodulin-dependent kinase type II (CaMKII) and calcineurin (CaN). While the role of CaMKII in targeting the APC/C inhibitor XErp1/Emi2 for destruction is well-established, it remained elusive how CaN affects APC/C-Cdc20 activation. Here, we discover that CaN contributes to APC/C-Cdc20 activation in Xenopus laevis oocytes by two independent but interrelated mechanisms. First, it facilitates the degradation of XErp1 by dephosphorylating it at a site that is part of a phosphorylation-dependent recruiting motif for PP2A-B ' 56, which antagonizes inhibitory phosphorylation of XErp1. Second, it dephosphorylates Cdc20 at an inhibitory site, thereby supporting its APC/C-activating function. Thus, our comprehensive analysis reveals that CaN contributes to timely APC/C activation at fertilization by both negatively regulating the APC/C inhibitory activity of XErp1 and positively regulating the APC/C-activating function of Cdc20.
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