期刊
EMBO REPORTS
卷 20, 期 1, 页码 -出版社
WILEY
DOI: 10.15252/embr.201845946
关键词
bacterial division; biomolecular condensation; droplet microfluidics; macromolecular crowding; phase separation
资金
- Fondo Europeo de Desarrollo Regional (FEDER)
- Agencia Estatal de Investigacion (AEI)
- Spanish government [BFU2014-52070-C2-2-P, BFU2016-75471-C2-1-P]
- National Institutes of Health [GM61074]
- National Science Foundation [MCB-1715984]
- European Social Fund [ESF 2014-2020]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM061074] Funding Source: NIH RePORTER
Macromolecular condensation resulting from biologically regulated liquid-liquid phase separation is emerging as a mechanism to organize intracellular space in eukaryotes, with broad implications for cell physiology and pathology. Despite their small size, bacterial cells are also organized by proteins such as FtsZ, a tubulin homolog that assembles into a ring structure precisely at the cell midpoint and is required for cytokinesis. Here, we demonstrate that FtsZ can form crowding-induced condensates, reminiscent of those observed for eukaryotic proteins. Formation of these FtsZ-rich droplets occurs when FtsZ is bound to SlmA, a spatial regulator of FtsZ that antagonizes polymerization, while also binding to specific sites on chromosomal DNA. The resulting condensates are dynamic, allowing FtsZ to undergo GTP-driven assembly to form protein fibers. They are sensitive to compartmentalization and to the presence of a membrane boundary in cell mimetic systems. This is a novel example of a bacterial nucleoprotein complex exhibiting condensation into liquid droplets, suggesting that phase separation may also play a functional role in the spatiotemporal organization of essential bacterial processes.
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