期刊
EMBO JOURNAL
卷 38, 期 5, 页码 -出版社
WILEY
DOI: 10.15252/embj.2018100323
关键词
Brucella; lipopolysaccharide; outer membrane; unipolar growth
资金
- Fonds de la Recherche Scientifique-Fonds National de la Recherche Scientifique (FRS-FNRS) [PDR T.0053.13, PDR Brucell-cycle T.0060.15, CDR J.0091.14]
- Concerted Research Actions of the Federation Wallonie-Bruxelles [17/22-087]
- UNamur
- Ph.D. grant from FRIA (FRS-FNRS)
- European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program [693630]
- FRS-FNRS
- FNRS-WELBIO [WELBIO-CR-2015A-05]
- Research Department of the Communaute Francaise de Belgique (Concerted Research Action)
The zoonotic pathogen Brucella abortus is part of the Rhizobiales, which are alpha-proteobacteria displaying unipolar growth. Here, we show that this bacterium exhibits heterogeneity in its outer membrane composition, with clusters of rough lipopolysaccharide co-localizing with the essential outer membrane porin Omp2b, which is proposed to allow facilitated diffusion of solutes through the porin. We also show that the major outer membrane protein Omp25 and peptidoglycan are incorporated at the new pole and the division site, the expected growth sites. Interestingly, lipopolysaccharide is also inserted at the same growth sites. The absence of long-range diffusion of main components of the outer membrane could explain the apparent immobility of the Omp2b clusters, as well as unipolar and mid-cell localizations of newly incorporated outer membrane proteins and lipopolysaccharide. Unipolar growth and limited mobility of surface structures also suggest that new surface variants could arise in a few generations without the need of diluting pre-existing surface antigens.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据