期刊
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
卷 167, 期 -, 页码 122-129出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ecoenv.2018.09.127
关键词
Arylamidase; Ochrobactrum sp PP-2; Propanil; 3 4-dichloroaniline; Biodegradation
资金
- National Natural Science Foundation of China [31670111]
- National Key Research and Development Program of China [2016YFD0800203]
Propanil, one of the most extensively used post-emergent contact herbicides, has also been reported to have adverse effect on environmental safety. A bacterial strain of Ochrobactrum sp. PP-2, which was capable of transforming propanil, was isolated from a propanil-contaminated soil collected from a chemical factory. An arylamidase gene mah responsible for transforming propanil to 3,4-dichloroaniline (3,4-DCA) was cloned from strain PP-2 by shotgun method and subsequently confirmed by function expression. The arylamidase Mah shares low amino acid sequence identity (27-50%) with other biochemically characterized amidases and shows less than 30% identities to other reported propanil hydrolytic enzymes. Mah was most active at pH 8 and 35 degrees C. Mah had a remarkable activity toward propanil (K-m = 6.3 +/- 1.2 mu M), showing the highest affinity efficiency for propanil as compared with other reported propanil hydrolytic enzymes. Our study also provides a new arylamidase for the hydrolysis of propanil.
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