期刊
DISEASE MODELS & MECHANISMS
卷 12, 期 1, 页码 -出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dmm.029462
关键词
CRISPR; Genome editing; Mouse; Mutagenesis
资金
- NIH HHS [U42 OD012210, UM1 OD023221] Funding Source: Medline
Over the past decade, new methods and procedures have been developed to generate genetically engineered mouse models of human disease. This At a Glance article highlights several recent technical advances in mouse genome manipulation that have transformed our ability to manipulate and study gene expression in the mouse. We discuss how conventional gene targeting by homologous recombination in embryonic stem cells has given way to more refined methods that enable allele-specific manipulation in zygotes. We also highlight advances in the use of programmable endonucleases that have greatly increased the feasibility and ease of editing the mouse genome. Together, these and other technologies provide researchers with the molecular tools to functionally annotate the mouse genome with greater fidelity and specificity, as well as to generate new mouse models using faster, simpler and less costly techniques.
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