期刊
DEVELOPMENT
卷 146, 期 2, 页码 -出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.169235
关键词
Liver development; Ubiquitin-like proteins; Knockout mouse; Cdk5rap3; Ufmylation; ER stress
资金
- Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences [2016-I2M-3-002, 2017-I2M-1-008]
- National Key Research and Development Program of China [2016YFA0100103]
- National Natural Science Foundation of China [31501176, 91231111]
Protein modification by ubiquitin and ubiquitin-like proteins (UBLs) regulates numerous biological functions. The UFM1 system, a novel UBL conjugation system, is implicated in mouse development and hematopoiesis. However, its broad biological functions and working mechanisms remain largely elusive. CDK5RAP3, a possible ufmylation substrate, is essential for epiboly and gastrulation in zebrafish. Herein, we report a crucial role of CDK5RAP3 in liver development and hepatic functions. Cdk5rap3 knockout mice displayed prenatal lethality with severe liver hypoplasia, as characterized by delayed proliferation and compromised differentiation. Hepatocyte-specific Cdk5rap3 knockout mice suffered post-weaning lethality, owing to serious hypoglycemia and impaired lipid metabolism. Depletion of CDK5RAP3 triggered endoplasmic reticulum stress and activated unfolded protein responses in hepatocytes. We detected the in vivo interaction of CDK5RAP3 with UFL1, the defined E3 ligase in ufmylation. Notably, loss of CDK5RAP3 altered the ufmylation profile in liver cells, suggesting that CDK5RAP3 serves as a novel substrate adaptor for this UBL modification. Collectively, our study identifies CDK5RAP3 as an important regulator of ufmylation and suggests the involvement of ufmylation in mammalian development.
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