期刊
DENTAL MATERIALS
卷 34, 期 12, 页码 1783-1796出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dental.2018.09.015
关键词
Phenylbis(acyl) phosphine oxides; Diphenyl(acyl) phosphine oxides; Camphorquinone; Cytotoxicity; Genotoxicity
资金
- Deutsche Forschungsgemeinschaft/German National Science Foundation (DFG) [VO 1727/1-2, VO 1727/1-4]
Objectives. Phenylbis(acyl) phosphine oxide (BAPO) and diphenyl(acyl) phosphine oxide (TPO) are alternative photoinitiators to camphorquinone (CQ) in dental resinous materials. Aim of this study was to investigate their cytotoxic/genotoxic potential in human oral keratinocytes (OKF6/Tert2) and Chinese hamster lung fibroblasts (V79) in comparison to CQ. Methods. Cells were exposed to different concentrations of BAPO and TPO (1-50 mu M). Cytotoxicity was evaluated using H33342 and MTT assay, cell proliferation by BrdU proliferation assay and microscopy. Effects on cellular redox homeostasis were assessed by detecting intracellular levels of reactive oxygen/nitrogen species (ROS/RNS) using the DCFH2 assay and by quantification of mRNA expression of oxidatively regulated, cyto-protective enzymes. Genotoxic potential was determined by use of micronucleus (MN) assay. Results. BAPO and TPO induced a concentration-dependent decrease of cell number. BAPO and TPO showed 50- to 250-fold higher cytotoxicity than CQ. In contrast to CQ both photoinitiators revealed no increase of intracellular ROS/RNS. However, BAPO (10 mu M) at least significantly induced mRNA-expression of redox-regulated proteins after 24h similar to 2.5 mM CQ. Additionally, BAPO significantly raised the number of micronuclei, but only in V79 cells (10 mu M: 12 +/- 1, 2.5 mM CQ: 15 +/- 1, medium control: 6 +/- 3). However, it also significantly decreased proliferation of these cells (10 mu M BAPO: 19.8% +/- 17.3% compared to controls). Significance. BAPO and TPO revealed concentration-dependent cytotoxic effects in human oral keratinocytes and V79 cells. However, in contrast to CQ no generation of intracellular ROS/RNS was found. Only BAPO induced genotoxicity in V79 cells. (C) 2018 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved.
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