4.6 Article

Hydrophobicity of graphene as a driving force for inhibiting biofilm formation of pathogenic bacteria and fungi

期刊

DENTAL MATERIALS
卷 35, 期 3, 页码 403-413

出版社

ELSEVIER SCI LTD
DOI: 10.1016/j.dental.2018.09.016

关键词

Peri-implantitis; Coating; Implant; Candida albicans; Carbon; Infection; Surface free energy; Wettability; Roughness; Graphene

资金

  1. National Research Foundation, Prime Minister' Office, Singapore, under its Medium Sized Centre Program
  2. Singapore Ministry of Education, Singapore [R-221-000-091-112]
  3. National University Health System, Singapore (NUHS Open Collaborative Research Grant NUHS O-CRG 2016 Oct-25, NUHS Seed Grant Call FY2018 Sep-07, VR)
  4. National Medical Research Council Singapore [NMRC/CIRG/1408/2014]

向作者/读者索取更多资源

Objective. To evaluate the surface and wettability characteristics and the microbial biofilm interaction of graphene coating on titanium. Methods. Graphene was deposited on titanium (Control) via a liquid-free technique. The transfer was performed once (TiGS), repeated two (TiGD) and five times (TiGV) and charac-terized by AFM (n = 10), Raman spectroscopy (n =10), contact angle and SFE (n = 5). Biofilm formation (n =3) to Streptococcus mutans, Enterococcus faecalis, Pseudomonas aeruginosa and Candida albicans was evaluated after 24h by CV assay, CFU, XTT and confocal microscopy. Statistics were performed by one-way Anova, Tukey's tests and Pearson's correlation analysis at a pre-set significance level of 5 %. Results. Raman mappings revealed coverage yield of 82 % for TiGS and >= 99 % for TiGD and TiGV. Both TiGD and TiGV presented FWHM > 44 cm(-1) and I-D/I-G ratio < 0.12, indicating multiple graphene layers and occlusion of defects. The contact angle was significantly higher for TiGD and TiGV (110 degrees and 117 degrees) comparing to the Control (70 degrees). The SFE was lower for TiGD (13.8 mN/m) and TiGV (12.1 mN/m) comparing to Control (38.3 mN/m). TiGD was selected for biofilm assays and exhibited significant reduction in biofilm formation for all microorganisms compared to Control. There were statistical correlations between the high contact angle and low SFE of TiGD and decreased biofilm formation. Significance. TiGD presented high quality and coverage and decreased biofilm formation for all species. The increased hydrophobicity of graphene films was correlated with the decreased biofilm formation for various species. (C) 2018 Published by Elsevier Inc. on behalf of The Academy of Dental Materials.

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