期刊
CLINICAL ORAL INVESTIGATIONS
卷 23, 期 9, 页码 3571-3580出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00784-018-2779-1
关键词
Proteomics; Saliva; Periodontitis; Protein biomarker; Ingenuity Pathway Analysis
资金
- Bio & Medical Technology Development Program of the National Research Foundation (NRF) - Ministry of Science and ICT [NRF-2017M3A9B6062984]
Objectives Saliva is a bodily fluid transuded from gingival crevice fluid and blood and contains many proteins. Proteins in saliva have been studied as markers for periodontal diseases. Mass spectrometric analysis is applied to investigate biomarker proteins that are related to periodontitis. Material and methods Saliva samples were collected from 207 participants including 36 pairs matched for age, sex, and smoking who joined Yangpyeong health cohort. Periodontitis was defined by 2005 5th European guideline. Shotgun proteomics was applied to detect proteins from saliva samples. Principal component analysis and Ingenuity Pathway Analysis for canonical pathway and protein pathway were applied. Protein-protein interaction was also applied. Enzyme-linked immunosorbent assay (ELISA) was used to verify the candidate protein markers among another matched participants (n = 80). Results Shotgun proteomics indicated that salivary S100A8 and S100A9 were candidate biomarkers for periodontitis. ELISA confirmed that both salivary S100A8 and S100A9 were higher in those with periodontitis compared to those without periodontitis (paired-t test, p < 0.05). Conclusion Our proteomics data showed that S100A8 and S100A9 in saliva could be candidate biomarkers for periodontitis. The rapid-test-kit using salivary S100A8 and S100A9 will be a practical tool for reducing the risk of periodontitis and promotion of periodontal health.
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