期刊
CIRCULATION RESEARCH
卷 124, 期 1, 页码 94-100出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/CIRCRESAHA.118.313234
关键词
atherosclerosis; cholesterol; diet; fatty acids, volatile; feces; inflammation
资金
- CardioVasculair Onderzoek Nederland [CVON2012-03]
- Jan Kornelis de Cock Foundation
- Graduate School for Drug Exploration, University of Groningen
- Netherlands Organization for Scientific Research VIDI-grant [NWO-VIDI 864.13.013]
- Netherlands Organization for Scientific Research VIDI [917.15.350]
- University Medical Center Groningen
Rationale: Several studies have suggested a role for the gut microbiota in inflammation and atherogenesis. A causal relation relationship between gut microbiota, inflammation, and atherosclerosis has not been explored previously. Objective: Here, we investigated whether a proinflammatory microbiota from Caspase1(-/-) (Casp1(-/-)) mice accelerates atherogenesis in Ldlr(-/-)mice. Method and Results: We treated female Ldlr-/- mice with antibiotics and subsequently transplanted them with fecal microbiota from Casp1(-/-) mice based on a cohousing approach. Autologous transplantation of fecal microbiota of Ldlr(-/-) mice served as control. Mice were cohoused for 8 or 13 weeks and fed chow or high-fat cholesterol-rich diet. Fecal samples were collected, and factors related to inflammation, metabolism, intestinal health, and atherosclerotic phenotypes were measured. Unweighted Unifrac distances of 16S rDNA (ribosomal DNA) sequences confirmed the introduction of the Casp1(-/-) and Ldlr(-/-) microbiota into Ldlr(-/-) mice (referred to as Ldlr(-/-) (Casp1(-/-)) or Ldlr(-/-) ( Ldlr(-/-)) mice). Analysis of atherosclerotic lesion size in the aortic root demonstrated a significant 29% increase in plaque size in 13-week high-fat cholesterol-fed Ldlr-/-(Casp1-/-) mice compared with Ldlr(-/-) (Ldlr(-/-)) mice. We found increased numbers of circulating monocytes and neutrophils and elevated proinflammatory cytokine levels in plasma in high-fat cholesterol-fed Ldlr(-/-) (Casp1(-/-)) compared with Ldlr(-/-) (Ldlr(-/-)) mice. Neutrophil accumulation in the aortic root of Ldlr(-/-) (Casp1(-/-)) mice was enhanced compared with Ldlr(-/-) (Ldlr(-/-)) mice. 16S-rDNA-encoding sequence analysis in feces identified a significant reduction in the short-chain fatty acid-producing taxonomies Akkermansia, Christensenellaceae, Clostridium, and Odoribacter in Ldlr(-/-) (Casp1(-/-)) mice. Consistent with these findings, cumulative concentrations of the anti-inflammatory short-chain fatty acids propionate, acetate and butyrate in the cecum were significantly reduced in 13-week high-fat cholesterol-fed Ldlr(-/-) (Casp1(-/-)) compared with Ldlr(-/-)(Ldlr-/-) mice. Conclusions: Introduction of the proinflammatory Casp1(-/-) microbiota into Ldlr(-/-)mice enhances systemic inflammation and accelerates atherogenesis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据