期刊
CHROMATOGRAPHIA
卷 82, 期 1, 页码 101-110出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s10337-018-3647-5
关键词
Review; Proteomics research; Peptide mapping; Peptide profiling; Nano-LC
资金
- Flemish Agency of Innovation and Entrepreneurship (VLAIO) [IWT.150467]
- Research Foundation Flanders (FWO) [G 025916N, G033018N]
Liquid chromatography coupled with mass spectrometric detection is one of the major technologies used for protein sequencing, identification, and quantification. This review provides an introduction of the current state-of-the-art technology in peptide profiling using nano-liquid chromatography-mass spectrometry applied to large-scale protein or proteome analysis. In particular, different aspects of the bottom-up proteomics workflow are covered, including aspects of sample preparation such as protein digestion, nanoflow gradient reversed-phase chromatographic separation, LC-MS interfacing via electrospray ionization, tandem mass spectrometry of digests, protein identification via database searches, and finally peptide quantitation.
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