期刊
CHINESE CHEMICAL LETTERS
卷 29, 期 12, 页码 1815-1818出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cclet.2018.10.012
关键词
Nanoparticles; Shape; Exocytosis; Uptake; Transport
资金
- Science and Technology Commission of Shanghai Municipality [18ZR1404100]
- Shanghai Pujiang Program [18PJD001]
- National Natural Science Foundation of China [81573363, 81690263]
- Youth Teacher Training Program of Shanghai Municipal Education Commission [ZZJKYX16011]
- Shanghai Municipal Commission of Health and Family Planning [20164Y0213]
Nanoparticles are easily to be taken up by cells but hard to be transported across epithelia, which plays an important role in absorption and toxicity. This paper aims to elucidate the effect of nanoparticle shape on bilateral exocytosis of Caco-2 cells. The fluorescent resonance energy transfer (FRET) probes (DiO and Dil) were utilized to label nanospheres and nanorods for determining and tracking nanoparticles. The results showed that more nanorods were internalized into Caco-2 cells than nanospheres. But the apical exocytosis rate of nanospheres from Caco-2 cells was faster than that of nanorods significantly. In addition, only less than 2% of intact nanoparticles were transported across monolayers for both nanoparticles, but the exocytosis behaviors between them was absolutely different. Compared to nanospheres, nanorods preferred basolateral exocytosis to apical exocytosis. Therefore, the shape is a critical parameter in cellular uptake and transport, even intracellular fate, which should be primarily considered in design of oral nanoparticles. (C) 2018 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.
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