期刊
CHEMICAL RESEARCH IN TOXICOLOGY
卷 32, 期 1, 页码 90-99出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.chemrestox.8b00204
关键词
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资金
- Tennessee Board of Regents
- Tennessee Technological University
- Tennessee Technological University URECA! Grants Program
- Lipscomb University College of Pharmacy and Health Sciences
Our previous research has shown that alpha-(N)-heterocyclic thiosemicarbazone (TSC) metal complexes inhibit human topoisomerase II alpha (TopoII alpha), while the ligands without metals do not. To find out the structural elements of TSC that are important for inhibiting TopoII alpha, we have synthesized two series of alpha-(N)-heterocyclic TSCs with various substrate ring segments, side chain substitutions, and metal ions, and we have examined their activities in TopoII alpha-mediated plasmid DNA relaxation and cleavage assays. Our goal is to explore the structure-activity relationship of alpha-(N)-heterocyclic TSCs and their effect on TopoII alpha. Our data suggest that, similar to Cu(II)-TSCs, Pd(II)-TSC complexes inhibit plasmid DNA relaxation mediated by TopoII alpha. In TopoII alpha-mediated plasmid DNA cleavage assays, the Cu(II)-TSC complexes induce higher levels of DNA cleavage than their Pd(II) counterparts. The Cu(II)-TSC complexes with methyl, ethyl, and tert-butyl substitutions are slightly more effective than those with benzyl and phenyl groups. The alpha-(N)-heterocyclic ring substrates of the TSCs, including benzoylpyridine, acetylpyridine, and acetylthiazole, do not exhibit a significant difference in TopoII alpha-mediated DNA cleavage. Our data suggest that the metal ion of TSC complexes plays a predominant role in inhibition of TopoII alpha, the side chain substitution of the terminal nitrogen plays a secondary role, while the substrate ring segment has the least effect. Our molecular modeling data support the biochemical data, which together provide a mechanism by which Cu(II)-TSC complexes stabilize TopoII alpha-mediated cleavage complexes.
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