期刊
CANCER LETTERS
卷 440, 期 -, 页码 202-210出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2018.10.017
关键词
Adipose tissue-derived mesenchymal stem cells; Lung cancer; TRAIL; IFN-beta; Necrotic cell death
类别
资金
- Korea Health Technology R&D Project through the Korea Health Industry Development Institute - Ministry of Health & Welfare, Republic of Korea [HI17C1365]
- Basic Science Research program [NRF-2017R1C1B5017842]
- Small Grant for Exploratory Research (SGER) Program through the National Research Foundation of Korea - Korean government (Ministry of Education) [NRF-2017R1D1A1A02018088, NRF-2017R1D1A1A02019212]
Although mesenchymal stem cells (MSCs) have been reported to inhibit tumor growth, the mechanism controlling this tumor suppression function is unclear. Here, we report that high-density (40,000 cells/cm(2)) cultured adipose tissue-derived MSCs (40K-ASCs) expressed interferon (IFN)-beta and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL); we also found that serum deprivation during cell culture induced the expression of IFN-beta and TRAIL. In addition, the mRNA expression of IFN-beta, but not TRAIL, was increased during the washing step required for the transplantation of normal-density (5000 cells/cm(2)) cultured ASCs (5K-ASCs). When the human lung cancer cell line H460 was co-cultured with 40K-ASCs, necrotic cell death was dramatically increased in vitro. When ASCs were injected after four washes, both 5K-ASCs and 40K-ASCs substantially reduced tumor weight in H460-derived cancer animal models. These results suggest that serum deprivation during the culture of 40K-ASCs or during the washing step of 5K-ASCs can induce IFN-beta and/or TRAIL expression, ultimately leading to the tumor suppression capability of ASCs.
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