期刊
BULLETIN OF MATERIALS SCIENCE
卷 41, 期 5, 页码 -出版社
INDIAN ACAD SCIENCES
DOI: 10.1007/s12034-018-1650-y
关键词
Ferric oxide nanoparticles; peroxidase enzyme; enzyme mimic; CTAB
In the present work, pristine and cetyl trimethyl ammonium bromide (CTAB)-coated ferric oxide nanoparticles (CTAB@ Fe2O3 NPs) were synthesized and studied as enzyme mimics. The w/w ratio of Fe2O3 to CTAB was varied as 1: 1 and 1: 2. Transmission electron microscopic analysis revealed that pristine NPs had an average size of 50 nm, whereas the presence of CTAB resulted in the formation of nanorods with length of 130 nm. BET studies confirmed enhancement of surface area on CTAB coating, which was maximum for w/w ratio 1: 1. The synthesized pristine NPs and CTAB-coated NPs were evaluated for their peroxidase mimic activity using o-dianisidine dihydrochloride as substrate. Optimum pH, temperature, substrate and NPs concentration for the reaction were 1, 25 degrees C, 0.16 mg ml(-1) and 1 mg ml-1, respectively. Peroxidase mimic activity of CTAB@ Fe2O3 NPs (w/w 1: 1) was higher than that of pristine NPs. However, further increase in CTAB coating (w/w 1: 2) resulted in lowering of peroxidase mimic activity. Kinetic analysis was carried out at optimized conditions; maximum velocity (Vmax) and Michaelis constant (Km) value of CTAB@ Fe2O3 NPs at 1: 1 w/w ratio were 7.69 mM and 1.12 mu mol s(-1), respectively.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据