期刊
BMC PLANT BIOLOGY
卷 18, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s12870-018-1607-8
关键词
Leaf removal; Light transmission rate; Maize; Photosynthesis; TMT label
资金
- national key research and development program of China [2016YFD0300308]
- Natural Science Foundation of Jiangsu Province [BK20170720]
- China Postdoctoral Science Foundation [2017 M611832]
- Postdoctoral Science Foundation funded project of Jiangsu Province [1701040A]
- National Natural Science Foundation of China [31171497]
- National Basic Research Program of China (973 Program) [2011CB100105]
- National Food Science and Technology of High-yield Program of China [2011BAD16B09]
- Special Fund for Agro-scientific Research in the Public Interest of China [20120306]
- 111 Project [B16026]
BackgroundUnder high plant density, intensifying competition among individual plants led to overconsumption of energy and nutrients and resulted in an almost dark condition in the lower strata of the canopy, which suppressed the photosynthetic potential of the shaded leaves. Leaf removal could help to ameliorate this problem and increase crop yields. To reveal the mechanism of leaf removal in maize, tandem mass tags label-based quantitative analysis coupled with liquid chromatography-tandem mass spectrometry were used to capture the differential protein expression profiles of maize subjected to the removal of the two uppermost leaves (S-2), the four uppermost leaves (S-4), and with no leaf removal as control (S-0).ResultsExcising leaves strengthened the light transmission rate of the canopy and increased the content of malondialdehyde, whereas decreased the activities of superoxide dismutase and peroxidase. Two leaves removal increased the photosynthetic capacity of ear leaves and the grain yield significantly, whereas S-4 decreased the yield markedly. Besides, 239 up-accumulated proteins and 99 down-accumulated proteins were identified between S-2 and S-0, which were strongly enriched into 30 and 23 functional groups; 71 increased proteins and 42 decreased proteins were identified between S-4 and S-0, which were strongly enriched into 22 and 23 functional groups, for increased and decreased proteins, respectively.ConclusionsDifferent defoliation levels had contrastive effects on maize. The canopy light transmission rate was strengthened and proteins related to photosynthetic electron-transfer reaction were up-regulated significantly for treatment S-2, which improved the leaf photosynthetic capacity, and obtained a higher grain yield consequently. In contrast, S-4 decreased the grain yield and increased the expressions of proteins and genes associated with fatty acid metabolism. Besides, both S-2 and S-4 exaggerated the defensive response of maize in physiological and proteomic level. Although further studies are required, the results in our study provide new insights to the further improvement in maize grain yield by leaf removal.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据