期刊
BMC GENOMICS
卷 19, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s12864-018-5091-1
关键词
Transcriptional defect; Reprogramming barrier; SCNT embryo; RNA-seq; Mouse
资金
- National Natural Science Foundation of China [31372273, 31201789, 31501906]
- Leading Talent Introduction and Cultivation Plan Project in Anhui Province Colleges and Universities [gwfxZD2016171, gxyqZD2016196]
- Anhui University Research Innovation Platform Team Project [[2015]49]
- Major Project of Biology Discipline Construction in Anhui Province (2014)
Background: Nuclear reprogramming reinstates totipotency or pluripotency in somatic cells by changing their gene transcription profile. This technology is widely used in medicine, animal husbandry and other industries. However, certain deficiencies severely restrict the applications of this technology. Results: Using single-embryo RNA-seq, our study provides complete transcriptome blueprints of embryos generated by cumulus cell (CC) donor nuclear transfer (NT), embryos generated by mouse embryonic fibroblast (MEF) donor NT and in vivo embryos at each stage (zygote, 2-cell, 4-cell, 8-cell, morula, and blastocyst). According to the results from further analyses, NT embryos exhibit RNA processing and translation initiation defects during the zygotic genome activation (ZGA) period, and protein kinase activity and protein phosphorylation are defective during blastocyst formation. Two thousand three constant genes are not able to be reprogrammed in CCs and MEFs. Among these constant genes, 136 genes are continuously mis-transcribed throughout all developmental stages. These 136 differential genes may be reprogramming barrier genes (RBGs) and more studies are needed to identify. Conclusions: These embryonic transcriptome blueprints provide new data for further mechanistic studies of somatic nuclear reprogramming. These findings may improve the efficiency of somatic cell nuclear transfer.
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