期刊
BMC CELL BIOLOGY
卷 19, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s12860-018-0181-0
关键词
Dormancy; Diapause; Daphnia; Cytoskeleton; Actin; Tubulin; Embryo development; Temperature; Early embryonic cell cycle
类别
资金
- Erasmus Plus program
- China Scholarship Council
- Research School Plus - DFG Excellence Initative [GSC 98/3]
- BBSRC Midlands Integrative Biosciences Training Partnership
BackgroundDiapause is a form of dormancy that is genetically predetermined to allow animals to overcome harsh environmental conditions. It is induced by predictive environmental cues bringing cellular activity levels into a state of suspended animation. Entering diapause requires organismal, molecular and cellular adaptation to severely reduced energy flows. Cells must therefore have evolved strategies that prepare them for periods with limited metabolic resources. However, changes that occur on the (sub-)cellular level have not been thoroughly described.ResultsWe investigated mitotic activity and we monitored cytoskeletal network changes in successive stages of diapausing and non-diapausing Daphnia magna embryos using (immuno-)fluorescent labeling. We find that embryos destined to diapause show a delayed and 2.5x slower mitotic activity in comparison to continuously developing embryos. Development is halted when D. magna embryos reach similar to 3500 cells, whereupon mitotic activity is absent and cytoskeletal components are severely reduced, rendering diapause cells compact and condensed.ConclusionIn the initiation phase of diapause, the slower cell division rate points to prolonged interphase duration, preparing the cells for diapause maintenance. During diapause, cytoskeletal depletion and cellular condensation may be a means to save energy resources. Our data provide insights into the sub-cellular change of diapause in Daphnia.
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