期刊
BIOTECHNOLOGY PROGRESS
卷 35, 期 2, 页码 -出版社
WILEY
DOI: 10.1002/btpr.2764
关键词
enzymes; thermally stable; soluble films; reagents; DNA amplification
资金
- Natural Sciences and Engineering Research Council of Canada
- Grand Challenges Canada
- Canadian Foundation for Innovation
- Ontario Ministry of Research and Innovation
Rolling circle amplification (RCA), polymerase chain reaction (PCR), and loop-mediated isothermal amplification (LAMP), are powerful tools that can be used for gene manipulation, pathogen detection, and infectious disease diagnostics. However, these techniques require trained personnel, as the pipetting steps involved can lead to contamination and, consequently, erroneous results. Furthermore, many of the reagents used in molecular biology are thermally labile and must be kept within a cold-chain. In this article, we present a simple and cost-effective method that allows molecular biology reagents to be thermally stabilized into ready-to-use mastermixes via drying in pullulan and trehalose films. Our experimental results demonstrate that this method is capable of preserving the activity of RCA, PCR, LAMP, ligase, polynucleotide kinase, and Klenow fragment mastermixes for at least 3 months at ambient conditions. Thus, stabilizing reagents via drying in pullulan and trehalose film may allow for a drastic reduction in the number of pipetting steps and the elimination of the need for a cold chain. (c) 2018 American Institute of Chemical Engineers Biotechnol. Prog., 35: e2764, 2019.
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