期刊
BIOSENSORS & BIOELECTRONICS
卷 129, 期 -, 页码 100-106出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.12.050
关键词
Electrochemiluminescence; Electrochemiluminecence resonance energy transfer; Surface plasmon resonance; Nanogears; Antibiotic
类别
资金
- National Natural Science Foundation of China [21365004]
- Key Research and Development Project of Guangxi [AB18126048]
- Specific Research Project of Guangxi for Research Bases and Talents [AD18126005]
- Guangxi Natural Science Foundation [2013GXNSFDA019006, 2016GXNSFBA380201, 2018GXNSFAA138086]
- Young and middle-aged teachers basic ability promotion project by Guangxi Education Department [KY2016YB134]
- State Key Laboratory of Analytical Chemistry for Life Science, Nanjing University [SKLACL1810]
A dual gears electrochemiluminecence (ECL) aptasensing strategy for multiple selective determination of kanamycin and neocycin was designed on the basis of the combination of kanamycin and neocycin induced dual gears conversion, the loading platform of metal-organic frameworks (MOFs), surface plasmon resonance (SPR) and ECL resonance energy transfer (ERET) between CdS QDs and AuNPs (or PtNPs). In the absence of target, the dual gears were off. Then the Bl-AuNP (gear B) and aptamer 1-PtNPs acted as signal quenching elements to quench ECL intensity due to ERET process. Upon addition of kanamycin, the aptamer 1-PtNPs were removed from the gear gradually, the ECL was enhanced due to SPR process between AuNPs and CdS QDs. After the incubation of aptamer 2, the dual gears were off' again and ECL intensity was decreased by ERET process between AuNPs and CdS QDs. In the presence of neomycin, dual gears were on again, the ECL signal was enhanced by SPR process between AuNPs and CdS QDs. Under optimal condition, the proposed aptasensor exhibited wide linear ranges of kanamycin (10(-10)-10(-6) M) and neomycin (10(-9)-10(-5) M), and relatively low detection limits to kanamycin (1.7 x 10(-11) M) and neomycin (3.5 x 10(-10) M). The developed aptasensor realized the multiple ECL detection of kanamycin and neomycin with single luminophore, and was successfully applied to the detection of kanamycin and neomycin in food samples.
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