期刊
BIOSENSORS & BIOELECTRONICS
卷 124, 期 -, 页码 59-65出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.10.023
关键词
Electrochemiluminescence; Resonance energy transfer; Concanavalin A; G-C3N4-COOH@Glu; BSA@MnO2-MWCNTs-COOH@Glu
类别
资金
- Shanghai Science and Technology Committee [17070503000, 18dz2308700]
- National Natural Science Foundation of China [21373138]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT_16R49]
- International Joint Laboratory on Resource Chemistry (IJLRC)
An electrochemiluminescence (ECL) analytical platform was initially proposed based on the electrochemiluminescence resonance energy transfer (ECL-RET) mechanism for ultrasensitive detection of Concanavalin A (Con A). In this protocol, the glucose functionalized carboxylic g-C3N4 nanosheets (g-C3N4-COOH@Glu) and MnO2 nanoparticles covered carboxylic multi-wall carbon nanotubes (BSA@MnO2-MWCNTs-COOH@Glu) were synthesized and acted as ECL-RET electron donor and acceptor, respectively. Herein, glucose was served as the recognition element for binding Con A and MWCNTs was utilized as the carrier materials for loading MnO2. When the quenching probe BSA@MnO2-MWCNTs-COOH@Glu was incubated onto the modified electrodes via the specific carbohydrate-Con A interaction, the ECL signals of g-C3N4-COOH@Glu which used S2O82- as its coreactant have drastically declined. Under optimum conditions, this biosensor performed a sensitive detection of the Con A ranging from 1 x 10(-5) to 1 x 10(4) ng/mL with a detection limit of 2.2 fg/mL (S/N = 3). Moreover, favorable analytical outcomes for detecion Con A in actual serum samples were obtained, exhibiting huge applications in clinical diagnosis of this assay.
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