期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 83, 期 4, 页码 684-694出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09168451.2018.1562879
关键词
Two-component signal transduction; PhoQ; EvgS; SafA; PDC sensor fold
类别
资金
- Institute for Fermentation, Osaka [L-25-3-03]
- Japan Society for the Promotion of Science [KAKENHI] [JP20248012, JP16K07681, JP17J02501]
- Institute for Frontier Life and Medical Sciences, Kyoto University [Joint Usage/Research Center Program]
The PhoQ/PhoP two-component signal transduction system is conserved in various Gram-negative bacteria and is often involved in the expression of virulence in pathogens. The small inner membrane protein SafA activates PhoQ in Escherichia coli independently from other known signals that control PhoQ activity. We have previously shown that SafA directly interacts with the sensor domain of the periplasmic region of PhoQ (PhoQ-SD) for activation, and that a D179R mutation in PhoQ-SD attenuates PhoQ activation by SafA. In this study, structural comparison of wild-type PhoQ-SD and D179R revealed a difference in the cavity (SD (sensory domain) pocket) found in the central core of this domain. This was the only structural difference between the two proteins. Site-directed mutagenesis of the residues surrounding the SD pocket has supported the SD pocket as a site involved in PhoQ activity. Furthermore, the SD pocket has also been shown to be involved in SafA-mediated PhoQ control.
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