期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 83, 期 3, 页码 463-471出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09168451.2018.1547105
关键词
ABCA1; cell migration; cell density; cholesterol; ERK
类别
资金
- Japan Agency for Medical Research and Development [Advanced Research and Development Programs for Medical Innovation]
- Japan Society for the Promotion of Science [JSPS KAKENHI] [18H02167, 18H05269, 25221203]
- Grants-in-Aid for Scientific Research [18H02167] Funding Source: KAKEN
Temporal and spatial changes of membrane lipid distribution in the plasma membrane are thought to be important for various cellular functions. ATP-Binding Cassette A1 (ABCA1) is a key lipid transporter for the generation of high density lipoprotein. Recently, we reported that ABCA1 maintains an asymmetric distribution of cholesterol in the plasma membrane. Here we report that ABCA1 suppresses cell migration by modulating signal pathways. ABCA1 knockdown in mouse embryonic fibroblasts accelerated cell migration and increased activation of Rac1 and its localization to detergent-resistant membranes. Phosphorylation of MEK and ERK also increased. Inhibition of Rac1 or MEK-ERK signals suppressed cell migration in ABCA1 knockdown cells. Because our experimental conditions for cell migration did not contain cholesterol or lipid acceptors for ABCA1, cellular cholesterol content was not changed. These data suggest that ABCA1 modulates cell migration via Rac1 and MEK-ERK signaling by altering lipid distribution in the plasma membrane.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据