期刊
BIOMEDICINE & PHARMACOTHERAPY
卷 109, 期 -, 页码 188-194出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.biopha.2018.10.077
关键词
Ficus carica; AMP-activated protein kinase; Diabetes mellitus; Gluconeogenesis; Phosphoenolpyruvate carboxykinase; Glucose-6-phosphatase
资金
- National Health and Family Planning Commission for Scientific Research [WKJ-FJ-13]
- Key Personnel Training Program for Young and Middle-aged Talents in Fujian Health System [2015-ZQN-ZD-23]
The aim of this study was to explore the possible mechanisms of Ficus carica leaf (FCL) extract in suppressing hepatic gluconeogenesis in diabetic mice. Diabetic mice (streptozotocin-induced) received 1 g/kg of FCL extract twice a day for 6 weeks. Fasting blood glucose levels were measured and a 2-h oral glucose tolerance test was conducted. AMP-activated protein kinase (AMPK), phosphoenolpyruvate carboxykinase (PEPCK), glucose-6-phosphatase (G6Pase), and peroxisome proliferator activated receptor-gamma coactivator-1 alpha (PGC-1 alpha) expression was examined. HepG2 hepatocytes were treated with FCL extract and an AMPK inhibitor (compound C) or agonist (AICAR), and PEPCK, G6pase, PGC-1 alpha, AMPK, forkhead transcription factor O1 (FOXO1), and hepatic nuclear factor 4 alpha (HNF4 alpha) expression was determined. The results showed that FCL extract inhibited the expression of PEPCK and G6Pase in the liver of diabetic mice and HepG2 hepatocytes. FCL extract activated AMPK and decreased PGC-1 alpha, HNF4 alpha, and FOXO1 expression. The AMPK inhibitor attenuated those effects through inhibiting gluconeogenesis, while the AMPK agonist partially enhanced gluconeogenesis. In conclusion, FCL extract inhibits hepatic gluconeogenesis via activation of AMPK and down-regulation of gluconeogenic enzymes.
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