期刊
BIOMARKERS
卷 24, 期 2, 页码 166-179出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/1354750X.2018.1531929
关键词
mTBI; sub-network enrichment analysis; gene set enrichment analysis; transcriptomics; head trauma
资金
- Technology Investment Fund by Defense Research and Development Canada (DRDC)
Context: Due to the wide use of improvised explosive devices during modern warfare, primary blast-derived mild traumatic brain injury (mTBI) has become a major medical condition in the military. With minimal visually identifiable symptoms, an effective molecular biomarker system is desirable. Objective: We assessed the potential of mammalian hair follicle miRNAs as an mTBI biomarker. Materials and methods: Due to their well-established roles in mTBI molecular pathology, the expression level of miR-183, miR-26a, miR-181c, miR-29a, miR-34a and miR-27b was determined using qRT-PCR in whisker hair follicles from rats subject to head-only exposure to a single-pulse shock wave. Based on established transcriptomics profiles, sub-network enrichment analysis (SNEA) was also conducted. Results: The results revealed that molecular networks involving miR-183, miR-26a and miR-181c were enriched in multiple treatments, whereas sub-networks of miR-29a, miR-34a and miR-27b were unique to individual exposure groups. Discussion: Our study showed that all six miRNAs were reflective of the mTBI signature involved in cellular responses. Noteworthy was that the decrease in the transcript levels of miR-181c was correlated with shockwave exposure severity. Conclusion: This study demonstrates for the first time that mammalian hair follicles are capable of housing miRNA biomarkers for TBI.
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