期刊
BIOMACROMOLECULES
卷 19, 期 11, 页码 4286-4298出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.biomac.8b01163
关键词
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资金
- NSF Designing Materials to Revolutionize and Engineer our Future (DMREF) program [DMR-1234161, DMR-1235084]
- Penn Laboratory for Research on the Structure of Matter [NSF DMR-1120901]
- NSF [CHE 1709518, ACI-1053575, TG-CHE110041, CHE1213728]
- Deutsche Forschungsgemeinschaft
- UD COBRE NIH-COBRE [1P30 GM110758]
- Delaware INBRE grant [P20 GM103446]
- Northwestern University
- E.I. DuPont de Nemours Co.
- Dow Chemical Company
- DOE Office of Science by Argonne National Laboratory [DE-AC02-06CH11357]
- National Science Foundation [0960140]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [P30GM110758] Funding Source: NIH RePORTER
Computationally designed peptides form desired antiparallel, tetrameric coiled-coil bundles that hierarchically assemble into a variety of well-controlled nanostructures depending on aqueous solution conditions. The bundles selectively self-assemble into different structures: nanotubes, platelets, or needle-like structures at solution pH values of 4.5, 7, and 10, respectively. The self-assembly produces hollow tubes or elongated needle like structures at pH conditions associated with charged bundles (pH 4.5 or 10); at neutral pH, near the pI of the bundle, a plate-like self-assembled structure forms. Transmission electron microscopy and small-angle X-ray scattering show the nanotubes to be uniform with a tube diameter of similar to 13 nm and lengths of up to several mu m, yielding aspect ratios >1000. Combining the measured nanostructure geometry with the apparent charged states of the constituent amino acids, a tilted-bundle packing model is proposed for the formation of the homogeneous nanotubes. This work demonstrates the successful use of assembly pathway control for the construction of nanostructures with diverse, well- structured morphologies associated with the folding and self-association of a single type of molecule.
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