期刊
BIOCONJUGATE CHEMISTRY
卷 29, 期 11, 页码 3522-3526出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.bioconjchem.8b00680
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资金
- Cancer Prevention Research Institute of Texas [CPRIT RR170014]
- Robert A. Welch Foundation [C-1970]
Site-specific antibody conjugates with a well-defined structure and superb therapeutic index are of great interest for basic research, disease diagnostics, and therapy. Here, we develop a novel proximity-induced antibody conjugation strategy enabling site-specific covalent bond formation between functional moieties and native antibodies without antibody engineering or additional UV/chemical treatment. A high conjugation efficiency and specificity was achieved with IgGs from different species and subclasses. The utility of this approach was demonstrated by site-specific conjugation of the small-molecule fluorophore to a native antibody and in vitro characterization of its activities.
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