期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 505, 期 4, 页码 1216-1222出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2018.10.027
关键词
Astrocyte; Neuron; Visual cortex; Mouse; In vivo two-photon Ca-2(+) imaging; Prazosin
资金
- Japan Society for the Promotion of Science (JSPS) KAKENHI [25117004, 25221001, 16H01328, 17H06312, 17K14931, 18H05116]
- Core Research for Evolutional Science and Technology (CREST)-Japan Agency for Medical Research and Development (AMED)
- Brain Mapping by Integrated Neurotechnologies for Disease Studies (Brain/MINDS)-AMED
- Strategic International Research Cooperative Program (SICP)-AMED
- Asahi Glass Foundation
- Takeda Science Foundation
- Japan Foundation of Institute for Neuropsychiatry
Astrocytes are known to contact with a great number of synapses and may integrate sensory inputs. In the ferret primary visual cortex, astrocytes respond to a visual stimulus with a delay of several seconds with respect to the surrounding neurons. However, in the mouse visual cortex, it remains unclear whether astrocytes respond to visual stimulations. In this study, using dual-color simultaneous in vivo two-photon calcium imaging of neurons and astrocytes in the awake mouse visual cortex, we examined the visual response of astrocytes and their precise response timing relative to the surrounding neurons. Neurons reliably responded to visual stimulations, whereas astrocytes often showed neuromodulator-mediated global activities, which largely masked small visual responses. Administration of the selective alpha 1-adrenergic receptor antagonist prazosin substantially reduced such global astrocytic activities without affecting the neuronal visual responses. In the presence of prazosin, astrocytes showed weak but consistent visual responses mostly at their somata. Cross-correlation analysis estimated that the astrocytic visual responses were delayed by approximately 5 seconds relative to the surrounding neuronal responses. In conclusion, our research demonstrated that astrocytes in the primary visual cortex of awake mice responded to visual stimuli with a delay of several seconds relative to the surrounding neurons, which may indicate the existence of a common mechanism of neuron-astrocyte communication across species. (C) 2018 Published by Elsevier Inc.
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