期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 505, 期 4, 页码 1043-1049出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2018.10.008
关键词
Oxysterol; Apoptosis; KI67; Annexin V; Cancer
资金
- Financiadora de Estudos e Projetos [FINEP - 01.10.00779.00]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico
- Instituto Nacional de Ciencia e Tecnologia em Medicina Regenerativa (INCT-Regenera)
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo [FAPESP - 13/10073-5, 16/21676-0]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [16/21676-0] Funding Source: FAPESP
Oxysterols are 27-carbon oxidation products of cholesterol metabolism. Oxysterols possess several biological actions, including the promotion of cell death. Here, we examined the ability of several oxysterols to induce short-term death in cancerous (human breast cancer and mouse skin melanoma cells) and non-cancerous (human endothelial cells and lung fibroblasts) cell lines. We determined cell viability, Ki67 expression, cell cycle regulation, and apoptosis after 24-h incubations with oxysterols. We found that different oxysterols had different effects on the studied parameters. Moreover, the effects depended on cell type and oxysterol concentration. Three cytotoxic oxysterols (7-ketocholesterol, cholestane-3 beta-5 alpha-6 beta-triol, and 5 alpha-cholestane-3 beta,6 beta-diol) inhibited the S phase and stimulated the G0/G1 or G2/M phases. These oxysterols promoted apoptosis, determined with Annexin V and propidium iodide assays. These results showed that different oxysterols have cytotoxic effects depending on the cell line. The findings suggest a potential pharmacological utility of cytotoxic oxysterols. (C) 2018 Elsevier Inc. All rights reserved.
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