期刊
MITOCHONDRIAL DNA PART A
卷 28, 期 3, 页码 375-376出版社
TAYLOR & FRANCIS LTD
DOI: 10.3109/19401736.2015.1126829
关键词
Chloroplast genome; female; male; next-generation sequencing; Silene latifolia
In this study, we assembled two individuals' complete chloroplast genomes with one male and one female from the dioecious plant species white campion (Silene latifolia). The two chloroplast genomes have an identical composition with each other as a circular molecule of 150 931 bp in length, with an overall GC content of 36.4%. The genomes consist of a pair of inverted repeats (IRs) of 25 503 bp, separated by a large single-copy (LSC) region and a small single-copy (SSC) region of 82 708 and 17 217 bp, respectively. The genomes contain 111 single copy genes, including 77 protein-coding genes, 4 ribosomal RNA genes and 30 transfer RNA genes. In these protein-coding genes, eight genes (rpl16, rpoC1, rps16, petD, petB, ndhB, ndhA and atpF) contain a single intron and three genes (rps12, clpP and ycf3) contain two introns. The two newly sequenced chloroplast genomes provide valuable information for detecting polymorphisms within species and between sexes.
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