期刊
ANTICANCER RESEARCH
卷 38, 期 11, 页码 6171-6180出版社
INT INST ANTICANCER RESEARCH
DOI: 10.21873/anticanres.12970
关键词
Casein kinase 2; gastric cancer; CX-4945; biomarker; targeted agent
类别
资金
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Science, ICT & Future Planning [2017R1A2B2005772]
- Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) - Ministry of Health & Welfare, Republic of Korea [HI13C2096]
Background/Aim: Casein kinase 2 (CK2) is involved in multiple cellular processes. Furthermore, its overexpression in several human cancers has been associated with tumor progression. In this study, we evaluated the efficacy of the CK2 inhibitor, CX-4945, in gastric cancer cell lines and explored the potential predictive biomarkers for CX-4945 sensitivity. Materials and Methods: The sensitivity to CX-4945 was screened in 49 gastric cancer cell lines by the MTT assay. The mRNA and protein expression of CK2 subunits (alpha and alpha') were determined using qRT-PCR and western blot. Furthermore, the activity of CK2 alpha was measured by ELISA. Gene expression and mutations were analyzed via whole-exome and RNA sequencing. Results: The sensitivity to CX-4945 was determined by the inhibition rate (%) at the effective dose (10 mu M) which ranged from -1% to 89% in 49 gastric cancer cell lines. CK2 alpha', but not CK2 alpha, mRNA expression was correlated with CX-4945 sensitivity. Conclusion: In this study, CX-4945 showed modest antitumor efficacy in gastric cancer cell lines. CK2 might represent a potential therapeutic target for gastric cancer.
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