期刊
ANNALS OF APPLIED BIOLOGY
卷 174, 期 1, 页码 92-105出版社
WILEY
DOI: 10.1111/aab.12476
关键词
bacterial plant diseases; Lactobacillus plantarum; Pseudomonas syringae pv. actinidiae; Xanthomonas arboricola pv. pruni; Xanthomonas fragariae
资金
- Universitat de Girona [MPCUdG2016]
- Department d'Economia i Coneixement-ACCIO Catalonia [SGR 2014-697]
- Secretaria d'Universitats i Recerca, Departament d'Economia i Coneixement, Generalitat de Catalunya [2015 FI_B00515]
- European Union FP7 Food, Agriculture and Fisheries, Biotechnology [FP7-KBBE.2013.1.2-04 613678]
- European Fund for Economic and Regional Development (FEDER) of the European Union [AGL2015-69876-C2-1-R]
- Spain Ministerio de Economia y Competitividad
The use of lactic acid bacteria (LAB) to control multiple pathogens that affect different crops was studied, namely, Pseudomonas syringae pv. actinidiae in kiwifruit, Xanthomonas arboricola pv. pruni in Prunus and Xanthomonas fragariae in strawberry. A screening procedure based on in vitro and in planta assays of the three bacterial pathogens was successful in selecting potential LAB strains as biological control agents. The antagonistic activity of 55 strains was first tested in vitro and the strains Lactobacillus plantarum CC100, PM411 and TC92, and Leuconostoc mesenteroides CM160 and CM209 were selected because of their broad-spectrum activity. The biocontrol efficacy of the selected strains was assessed using a multiple-pathosystem approach in greenhouse conditions. L. plantarum PM411 and TC92 prevented all three pathogens from infecting their corresponding plant hosts. In addition, the biocontrol performance of PM411 and TC92 was comparable to the reference products (Bacillus amyloliquefaciens D747, Bacillus subtilis QST713, chitosan, acibenzolar-S-methyl, copper and kasugamycin) in semi-field and field experiments. The in vitro inhibitory mechanism of PM411 and TC92 is based, at least in part, on a pH lowering effect and the production of lactic acid. Moreover, both strains showed similar survival rates on leaf surfaces. PM411 and TC92 can easily be distinguished because of their different multilocus sequence typing and random amplified polymorphic DNA profiles.
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