期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 58, 期 2, 页码 561-565出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201811261
关键词
biocompatible reaction; mammalian cells; photocatalysis; uncaging reaction
资金
- National Basic Research Program of China [2014CB910300]
- National Natural Science Foundation of China [91632304, 21622207, 21472230, 81730095]
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDB20020200]
- Shanghai Committee of Science and Technology [18JC1420302]
The photo-manipulation of bioactive molecules provides unique advantages due to the high temporal and spatial precision of light. The first visible-light uncaging reaction by photocatalytic deboronative hydroxylation in live cells is now demonstrated. Using Fluorescein and Rhodamine derivatives as photocatalysts and ascorbates as reductants, transient hydrogen peroxides were generated from molecular oxygen to uncage phenol, alcohol, and amine functional groups on bioactive molecules in bacteria and mammalian cells, including neurons. This effective visible-light uncaging reaction enabled the light-inducible protein expression, the photo-manipulation of membrane potentials, and the subcellular-specific photo-release of small molecules.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据