期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 58, 期 6, 页码 1739-1743出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201813032
关键词
chemiluminescence; invivo imaging; molecular probe; NQO1
资金
- CRI project [2018R1A3B1052702]
- Basic Science Research Program of NRF in Korea [2017R1D1A1B03030062]
- NIH [GM103790A]
- Robert A. Welch Foundation [F-0018]
- Israel Science Foundation (ISF)
- Binational Science Foundation (BSF)
- German Israeli Foundation (GIF)
- National Research Foundation of Korea [2017R1D1A1B03030062] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Activatable (turn-on) probes that permit the rapid, sensitive, selective, and accurate identification of cancer-associated biomarkers can help drive advances in cancer research. Herein, a NAD(P)H:quinone oxidoreductase-1 (NQO1)-specific chemiluminescent probe1 is reported that allows the differentiation between cancer subtypes. Probe1 incorporates an NQO1-specific trimethyl-locked quinone trigger moiety covalently tethered to a phenoxy-dioxetane moiety through a para-aminobenzyl alcohol linker. Bio-reduction of the quinone to the corresponding hydroquinone results in a chemiluminescent signal. As inferred from a combination of invitro cell culture analyses and invivo mice studies, the probe is safe, cell permeable, and capable of producing a turn-on luminescence response in an NQO1-positive A549 lung cancer model. On this basis, probe1 can be used to identify cancerous cells and tissues characterized by elevated NQO1 levels.
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