期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 57, 期 49, 页码 15990-15994出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201808402
关键词
aromatic compounds; biosynthesis; carbon dioxide fixation; photosynthetic chain; shikimate pathway
资金
- Shanghai Sailing Program [17YF1410300]
- China Postdoctoral Science Foundation [2018T110391]
- National Natural Science Foundation of China [31800019, 21777098, 31570101]
Directing CO2 conversion using photosynthetic microorganisms offers a promising route to couple CO2 sequestration with petrochemical replacement. However, the low-flux shikimate pathway remains largely unexploited for the synthesis of valuable aromatics. In addition, it is unclear how an enhanced low-flux pathway would influence the photosynthetic chain. We created a powerful metabolic sink by introducing the 2-phenylethanol pathway and an artificial feedback-inhibition-resistant cassette to Synechococcus elongatus. More than 30% of the fixed carbon was redirected to the shikimate pathway for aromatic synthesis, and carbon fixation and O-2 evolution increased significantly. A self-remodeling mechanism of the photosynthetic chain was discovered, which accelerates electron transport and reduces energy waste. This study represents a significant step toward the industrial viability of CO2 conversion into aromatic compounds and provides design guidance for improving photosynthetic efficiency.
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