期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 58, 期 12, 页码 3992-3995出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201900255
关键词
artificial cell walls; H-2 production; self-assembly; single-cell nanoencapsulation
资金
- NSFC [21871069, 51873050, 51703043]
- China Postdoctoral Science Foundation [2015M571401]
Single-cell encapsulation has become an effective strategy in cell surface engineering; however, the constniction of cell wall-like layers that allow the switching of the inherent functionality of the engineered cell is still rare. In this study, we show a universal way to create an enzyme-modulated oxygen-consuming sandwich-like layer by using polydopamine, laccase, and tannic acid as building blocks, which then could generate an anaerobic microenvironment around the cell. This layer protected the encapsulated C. pyrenoidosa cell against external stresses and enabled it to switch from normal photosynthetic O-2 production to photobiological H-2 production. The layer showed an smaller effect on the PSII activity, which contributed a significant enhancement on the rate (0.32 mu mol H(2)h(-1) (nzgchlorophyll)(-1)) and the duration (7 d) of H-2 production. This strategy is expected to provide a pathway for modulating the functionality of cells and for breakthroughs in the development of green energy alternatives.
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