期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 57, 期 50, 页码 16286-16290出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201808750
关键词
cell division; liposomes; membranes; synthetic biology; vesicles
资金
- Federal Ministry of Education and Research of Germany
- Max Planck Society
- Gottfried Wilhelm Leibniz-Program of the DFG [SCHW716/8-1]
- DFG Collaborative Research Centre Spatiotemporal dynamics of bacterial cells [TRR174/2017]
- DFG fellowship through the Graduate School of Quantitative Biosciences Munich
- Center for NanoScience Munich
The bacterial Min protein system was encapsulated in giant unilamellar vesicles (GUVs). Using confocal fluorescence microscopy, we identified several distinct modes of spatiotemporal patterns inside spherical GUVs. For osmotically deflated GUVs, the vesicle shape actively changed in concert with the Min oscillations. The periodic relocation of Min proteins from the vesicle lumen to the membrane and back is accompanied by drastic changes in the mechanical properties of the lipid bilayer. In particular, two types of oscillating membrane-shape changes are highlighted: 1)GUVs that repeatedly undergo fission into two connected compartments and fusion of these compartments back into a dumbbell shape and 2)GUVs that show periodic budding and subsequent merging of the buds with the mother vesicle, accompanied by an overall shape change of the vesicle reminiscent of a bouncing ball. These findings demonstrate how reaction-diffusion-based protein self-organization can directly yield visible mechanical effects on membrane compartments, even up to autonomous division, without the need for coupling to cytoskeletal elements.
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