期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 58, 期 8, 页码 2331-2335出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201812628
关键词
circular dichroism; DNA; DNA structures; G-quadruplexes; X-ray diffraction
资金
- Singapore Ministry of Education [MOE2015-T2-1-092, MOE2012-T3-1-001]
- National Research Foundation Investigatorship [NRF-NRFI2017-09]
- Nanyang Technological University
Recently, we observed the first example of a left-handed G-quadruplex structure formed by natural DNA, named Z-G4. We analysed the Z-G4 structure and inspected its primary 28-nt sequence in order to identify motifs that convey the unique left-handed twist. Using circular dichroism spectroscopy, NMR spectroscopy, and X-ray crystallography, we revealed a minimal sequence motif of 12 nt (GTGGTGGTGGTG) for formation of the left-handed DNA G-quadruplex, which is found to be highly abundant in the human genome. A systematic analysis of thymine loop mutations revealed a moderate sequence tolerance, which would further broaden the space of sequences prone to left-handed G-quadruplex formation.
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