期刊
ANALYTICAL SCIENCES
卷 34, 期 10, 页码 1155-1161出版社
JAPAN SOC ANALYTICAL CHEMISTRY
DOI: 10.2116/analsci.18P124
关键词
Mercury(II) ion detection; copper nanoclusters; signal amplification; fluorescent biosensor
资金
- National Natural Science Foundation of China [21375046, 21605056]
- Science and Technology Development of Jilin Province [20180101292JC]
- Science Foundation for Distinguished Young Scholars of Jilin City [20166028]
- Key Laboratory of Fine Chemicals of Jilin Province
This paper describes a novel method for label-free mercury(II) ion detection based on exonuclease III-induced target signal recycling amplification using double-stranded DNA templated copper nanoclusters. The synthesized DNA-Cu nanoclusters were used with exonuclease III loop amplification technology for ultra-high sensitivity detection of mercury(II) ions, which were detected by significantly decreased fluorescence intensity. Under the optimal experimental conditions, there was a clear linear relationship between Hg2+ concentration in the range of 0.04 to 8 nM and fluorescence intensity. The detection limit for Hg2+ was 4 pM. In addition, the interference of other metal ions on the mercury(II) ion detection was also studied. To confirm the application of the fluorescent sensor, it was applied to determine the concentrations of mercury(II) ions in tap water, and the results showed that the method can be used to detect mercury(II) ions in water samples successfully.
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