期刊
ANALYTICAL CHEMISTRY
卷 91, 期 1, 页码 1157-1163出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.8b04935
关键词
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资金
- National Natural Science Foundation of China [21627807]
- National Institutes of Health [R44GM119584, 1R01AI122298, R01A1122932]
The combination of direct sampling ionization and miniature mass spectrometer presents a promising technical pathway of point-of-care analysis in clinical applications. In this work, a miniature mass spectrometry system was used for analysis of tissue samples. Direct tissue sampling coupled with extraction spray ionization was used with a home-built miniature mass spectrometer, Mini 12. Lipid species in tissue samples were well profiled in rat brain, kidney, and liver in a couple of minutes. By incorporating a photochemical (Paterno-Bitchi) reaction, fast identification of lipid C=C location was realized. Relative quantitation of the lipid C=C isomer was performed by calculating the intensity ratio C=C diagnostic product ions, by which FA 18:1 (Delta 9)/FA 18:1 (Delta 11) was found to change significantly in mouse cancerous breast tissue samples. Accumulation of 2-hydroxylglutarate in human glioma samples, not in normal brains, can also be easily identified for rapid diagnosis.
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