期刊
ANALYTICAL CHEMISTRY
卷 91, 期 2, 页码 1408-1415出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.8b04027
关键词
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资金
- National Key Research and Development Program of China [2016YFA0201300]
- National Natural Science Foundation of China [21475125, 21675146]
- Instrument Developing Project of the Chinese Academy of Sciences [YZ201666]
Cytochrome c (Cyt c) release and cellular pH change are two important mediators of apoptosis. Effective methods to regulate or monitor such two events are therefore highly desired for apoptosis research and cancer cell therapy. Herein, we exploited electrostimulation to regulate cellular Cyt c release and apoptosis process, and by designing and preparing a smart and efficient plasmonic nanorobot (with surface-modified Cyt c-specific aptamer and 4-mercaptobenzoic acid) that is capable of Cyt c capture and self-sensing, we achieved real-time SERS monitoring of dynamic Cyt c release and simultaneous cell acidification in apoptosis during electrostimulation. Distinctly different molecular stress responses in the two events for cancerous MCF-7 and HeLa cells and normal L929 cells were identified and revealed. The method and results are valuable and promising for apoptosis and Cyt c-mediated biology studies.
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