期刊
ANALYTICA CHIMICA ACTA
卷 1054, 期 -, 页码 128-136出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2018.12.018
关键词
Microcystin-LR; Immunosensor; Mimic enzyme; Copper hydroxide nanozyme; G-quadruplex/hemin DNAzyme
资金
- National Science Foundation [21475047, 21705051, 21874048]
- Science and Technology Planning Project of Guangdong Province [2016B030303010]
- Program for the Top Young Innovative Talents of Guangdong Province [2016TQ03N305]
- Foundation for High-level Talents in South China Agricultural University
Recently, mimic enzymes have obtained particular interest by their high activity, stability, and biocompatibility. In this work, by coupling copper hydroxide nanozyme and G-quadruplex/hemin DNAzyme to form a double-integrated mimic enzyme, a visual, sensitive and selective immunosensor was established to detect microcystin-LR (MC-LR). In this immunoassay, the microplates were modified with core-shell silica/nickel silicate as the substrate to capture MC-LR antigens. Then, Cu(OH)(2) nanocages with fine regulation were used as the label to capture the secondary antibody for immunoreaction and the DNA primer for propagation, followed by using hybridization chain reaction to amplify the DNA primer, thus numerous DNAzymes (G-quadruplex/hemin) can be formed on the surface of Cu(OH)(2) nanocages with the aid of hemin. Such double-integrated mimic enzyme including Cu(OH)(2) nanozymes and DNAzymes showed excellent peroxidase activity for the chromogenic reaction of 2,20-azinobis-(3-ethylbenzothiazoline- 6-sulfonic acid) (ABTS), which realized the visual detection of MC-LR in the range from 0.007 to 75 mu g/L with the detection limit of 6 ng/mL, and thus provided the probability for the portable assessment of MC-LR in real sample. (C) 2018 Elsevier B.V. All rights reserved.
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