期刊
ANALYTICA CHIMICA ACTA
卷 1053, 期 -, 页码 62-69出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2018.12.002
关键词
Biodegradable polymer; Enzymatic degradation; Polyesters; Lipase; Nanoparticle; Liquid chromatography
资金
- Netherlands Organisation for Scientific Research (NWO) [053.21.113]
Biodegradable polymeric materials may eventually replace biostable materials for medical applications, including therapeutic devices, scaffolds for tissue engineering, and drug-delivery vehicles. To further develop such materials, a more fundamental understanding is necessary to correlate parameters including chemical-composition distribution within a macromolecular structure with the final properties of the material, including particle-size. A wide variety of analytical techniques have been applied for the characterization of polymer materials, including hyphenated techniques such as comprehensive twodimensional liquid chromatography (LC x LC). In this context, we have investigated enzymatic degradation of polyester-based nanoparticles, both in-solution and by the use of an immobilized-enzyme reactor (IMER). We have demonstrated for the first time the implementation of such an IMER in a size-exclusion chromatography system for on-line degradation and subsequent analysis of the polymer degradation products. The effect of residence times ranging from 12 s to 4 min on polymer degradation was assessed. IMER-assisted degradation is much faster compared to in-solution degradation, which requires several hours to days, and opens the possibility to use such reactors in LC x LC modulation interfaces. (C) 2018 Elsevier B.V. All rights reserved.
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