期刊
BIOCHEMICAL JOURNAL
卷 473, 期 -, 页码 571-580出版社
PORTLAND PRESS LTD
DOI: 10.1042/BJ20150828
关键词
eIF2B; protein synthesis; ubiquitination; vanishing white matter
资金
- UK Biotechnology & Biological Sciences Research Council [BB/J007706/1]
- BBSRC [BB/J007706/1, BB/G008396/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/J007706/1, BB/G008396/1] Funding Source: researchfish
The eukaryotic translation initiation factor eIF2B is a multi-subunit complex with a crucial role in the regulation of global protein synthesis in the cell. The complex comprises five subunits, termed alpha through epsilon in order of increasing size, arranged as a heterodecamer with two copies of each subunit. Regulation of the co-stoichiometric expression of the eIF2B subunits is crucial for the proper function and regulation of the eIF2B complex in cells. We have investigated the control of stoichiometric eIF2B complexes through mutual stabilization of eIF2B subunits. Our data show that the stable expression of the catalytic eIF2B epsilon subunit in human cells requires co-expression of eIF2B gamma. Similarly, stable expression of eIF2B delta requires both eIF2B beta and eIF2B gamma + epsilon. The expression of these subunits decreases despite there being no change in either the levels or the translation of their mRNAs. Instead, these subunits are targeted for degradation by the ubiquitin-proteasome system. The data allow us to propose a model for the formation of stoichiometric eIF2B complexes which can ensure their stoichiometric incorporation into the holocomplex.
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