Sex-specific plasticity of reproductive allocation in response to water depth in a clonal, dioecious macrophyte

Premise of the Study Sex-specific differences in reproductive investment contribute to sexual dimorphism in dioecious plants. Along environmental gradients, males and females may plastically adjust reproductive allocation differently because of contrasting reproductive costs. In dioecious macrophytes, variation in water depth is likely to influence reproductive allocation but has not been investigated in detail. Methods Vallisneria spinulosa was grown in aquatic mesocosms at water depths of 50, 100 and 150 cm for 14 weeks. Plasticity in allocation was measured to investigate whether sexual dimorphism in reproductive allocation and vegetative growth changed in response to varying water depths. Key Results Females invested a higher fraction of resources to sexual reproduction than males across all water depths and decreased proportional allocation to sexual structures in shallow and deep water compared to intermediate water depth. In contrast, males maintained similar sexual allocation across all water depths. Females displayed larger vegetative size than males, despite greater sexual investment, but decreased vegetative biomass more than males in shallow or deep water. The sexes invested similarly in clonal propagation by tubers at all water depths, but a trade-off with sexual reproduction was only evident in females. Conclusions Our results suggest that females of V. spinulosa have mechanisms to compensate for the costs of sexual reproduction in heterogeneous environments. Compared to males, females expressed greater plasticity in biomass allocated to sexual reproduction and vegetative growth in response to water depth variation. Environmental variation in underwater light availability probably caused the sex-specific allocation strategies found in V. spinulosa.