期刊
ADVANCED MATERIALS
卷 31, 期 7, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adma.201806380
关键词
aptamers; biomaterials; biomimetics; controlled release; growth factor delivery; mechanobiology
类别
资金
- Wellcome Trust [109838/Z/15/Z]
- Engineering and Physical Science Research Council [EP/R041628/1]
- Department of Bioengineering at Imperial College London
- TECNIOSpring PLUS postdoctoral fellowship part of the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie [712949]
- EPSRC [EP/R041628/1] Funding Source: UKRI
- Wellcome Trust [109838/Z/15/Z] Funding Source: Wellcome Trust
Biomaterial scaffolds that are designed to incorporate dynamic, spatiotemporal information have the potential to interface with cells and tissues to direct behavior. Here, a bioinspired, programmable nanotechnology-based platform is described that harnesses cellular traction forces to activate growth factors, eliminating the need for exogenous triggers (e.g., light), spatially diffuse triggers (e.g., enzymes, pH changes), or passive activation (e.g., hydrolysis). Flexible aptamer technology is used to create modular, synthetic mimics of the Large Latent Complex that restrains transforming growth factor-beta 1 (TGF-beta 1). This flexible nanotechnology-based approach is shown here to work with both platelet-derived growth factor-BB (PDGF-BB) and vascular endothelial growth factor (VEGF-165), integrate with glass coverslips, polyacrylamide gels, and collagen scaffolds, enable activation by various cells (e.g., primary human dermal fibroblasts, HMEC-1 endothelial cells), and unlock fundamentally new capabilities such as selective activation of growth factors by differing cell types (e.g., activation by smooth muscle cells but not fibroblasts) within clinically relevant collagen sponges.
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