期刊
ADVANCED FUNCTIONAL MATERIALS
卷 29, 期 4, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adfm.201806899
关键词
bioinspired scaffold; electrospun; hydrogel; microfibers; spinal cord injury
类别
资金
- National Key Research and Development Program of China [2016YFC1101505]
- National Natural Science Foundation of China [81772312, 51873107, 81601891]
- Shanghai Municipal Education Commission-Gaofeng Clinical Medicine Grant Support [20171906]
- Science and Technology Commission of Shanghai Municipality [18ZR1434200]
- Shanghai Jiao Tong University Medical and Research Program [ZH2018ZDA04]
- Shanghai talent development fund [2018099]
- Standardized Diagnosis and Treatment Project of Key Diseases in Jiangsu Province [BE2015641]
- Key Talented Man Project of Jiangsu Province [RC2011102]
- Shanghai municipal health and Family Planning Commission [201840027]
- Norte Portugal Regional Operational Programme (NORTE 2020), under the European Regional Development Fund (ERDF) [NORTE-01-0145-FEDER-000012]
- FEDER - Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020 -Operacional Programme for Competitiveness and Internationalisation (POCI), Portugal 2020
- Portuguese funds through FCT - Fundacao para a Ciencia e a Tecnologia/Ministerio da Ciencia, Tecnologia e Ensino Superior of Institute for Research and Innovation in Health Sciences [POCI-01-0145-FEDER-007274]
- FCT - Fundacao para a Ciencia e a Tecnologia, Portugal [SFRH/BD/137946/2018]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/137946/2018] Funding Source: FCT
Fully simulating the components and microstructures of soft tissue is a challenge for its functional regeneration. A new aligned hydrogel microfiber scaffold for spinal cord regeneration is constructed with photocrosslinked gelatin methacryloyl (GelMA) and electrospinning technology. The directional porous hydrogel fibrous scaffold consistent with nerve axons is vital to guide cell migration and axon extension. The GelMA hydrogel electrospun fibers soak up water more than six times their weight, with a lower Young's modulus, providing a favorable survival and metabolic environment for neuronal cells. GelMA fibers further demonstrate higher antinestin, anti-Tuj-1, antisynaptophysin, and anti-CD31 gene expression in neural stem cells, neuronal cells, synapses, and vascular endothelial cells, respectively. In contrast, anti-GFAP and anti-CS56 labeled astrocytes and glial scars of GelMA fibers are shown to be present in a lesser extent compared with gelatin fibers. The soft bionic scaffold constructed with electrospun GelMA hydrogel fibers not only facilitates the migration of neural stem cells and induces their differentiation into neuronal cells, but also inhibits the glial scar formation and promotes angiogenesis. Moreover, the scaffold with a high degree of elasticity can resist deformation without the protection of a bony spinal canal. The bioinspired aligned hydrogel microfiber proves to be efficient and versatile in triggering functional regeneration of the spinal cord.
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