期刊
ACS CHEMICAL NEUROSCIENCE
卷 10, 期 1, 页码 528-536出版社
AMER CHEMICAL SOC
DOI: 10.1021/acschemneuro.8b00406
关键词
Alzheimer's disease; amyloid precursor protein; amyloid-beta; ganglioside; Hp-s1; Hp-s1A; neurotrophic signaling; amyloidogenesis; neuritogenesis
资金
- Ministry of Science and Technology, Taiwan [MOST106-2113-M-033-005, MOST105-2320-B-038-049]
Alzheimer's disease (AD) is characterized by extracellular deposition of amyloid plaques, which are predominantly composed of amyloid-beta (A beta) peptide derived from amyloid precursor protein (APP) cleavage. APP interacts with tropomyosin receptor kinase A, a neurotrophic receptor associated with gangliosides and mediating neuronal survival and differentiation through the extracellular signal regulated protein kinase (ERK) pathway. The ganglioside Hp-s1's analogue Hp-s1A exerts neuritogenic activity; however, its effect on AD pathology remains unknown. To test the hypothesis that Hp-s1A is a potential candidate to treat AD, we established the AD-modeled cell line by expressing human Swedish and Indiana APP gene (APP-Swe/Ind) in N2a mouse neuroblastoma cells. The cells were treated with Hp-s1A or monosialoganglioside GM1 for comparison. The AD model cells expressing APP-Swe/Ind exhibited a significant reduction in viability, as well as neurite outgrowth rate, in comparison to the control cells expressing APP-695. APP C-terminal fragment-beta (CTF beta) and A beta 42 were increased in the AD cell lysates and the culture media, respectively. With the treatment of either Hp-slA or GM1 at 1 mu M, the AD model cells showed a significant increase in viability; however, only Hp-s1A reduced CTF beta levels in these cells. Further analysis of the culture media revealed that Hp-s1A also reduced A beta 42 production from AD model cells. The phosphorylation of ERK was elevated and the neurite outgrowth rate was restored with Hp-s1A treatment. In conclusion, the ganglioside analogue Hp-s1A inhibited amyloidogenic processing of APP and promoted neurotrophic activity and survival of AD model cells. Hp-s1A has great potential in AD therapeutic development.
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