期刊
ACS APPLIED MATERIALS & INTERFACES
卷 10, 期 47, 页码 40452-40459出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.8b16736
关键词
immobilization; proteins; surface chemistry; photochemistry; monolayers
资金
- Air Force Office of Scientific Research [AFOSR FA9550-16-1-0150]
- National Cancer Institute of the National Institutes of Health [U54CA199091]
- International Postdoctoral Exchange Fellowship Program 2016 from the Office of China Postdoctoral Council
- NIH/NCI [5T32CA186897-02]
- American Cancer Society-2017 Seattle Gala Paddle Raise Postdoctoral Fellowship [PF-18-118-01-CDD]
- NSF Graduate Research Fellowship [DGE-1324585]
- Soft and Hybrid Nanotechnology Experimental (SHyNE) Resource (NSF) [NNCI-1542205]
- State of Illinois
- International Institute for Nanotechnology (IIN)
- NATIONAL CANCER INSTITUTE [U54CA199091, T32CA186897] Funding Source: NIH RePORTER
This article describes a photochemical approach for independently patterning multiple proteins to an inert substrate, particularly for studies of cell adhesion. A photoactivatable chloropyrimidine ligand was employed for covalent immobilization of SnapTag fusion proteins on self-assembled monolayers of alkanethiolates on gold. A two-step procedure was used: first, patterned UV illumination of the surface activated protein capture ligands, and second, incubation with a SnapTag fusion protein bound to the surface in illuminated regions. Two different fluorescent proteins were patterned in registry with features of 400 nm in size over a 1 mm(2) area. An example is given wherein an anti-carcinoembryonic antigen (anti-CEA) scFv antibody was patterned to direct the selective attachment of a human cancer cell line that express the CEA antigen. This method enables the preparation of surfaces with control over the density and activity of independently patterned proteins.
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