期刊
ACS APPLIED MATERIALS & INTERFACES
卷 11, 期 9, 页码 8789-8796出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.8b20169
关键词
transparent liquid marble; embryonic stem cells (ESCs); embryonic bodies (EBs); one pot differentiation; nanoparticle-liquid interface
资金
- China Scholarship Council
- Monash Graduate Education
- Faculty of Engineering
- National Natural Science Foundation of China [U1732129]
- Monash Institute of Medical Engineering (MIME)
- Australian Research Council [IH170100009]
- Department of Industry, Innovation and Science (DIIS) of Victoria
- MyHealthTest Pty Ltd [ICG000457]
Transparent liquid marbles coated with hydrophobic silica nanoparticles were used as micro-bioreactors for embryonic stem cell (ESC) culturing. The high transparency of silica liquid marbles enables real-time and in situ monitoring of embryonic body (EB) formation and differentiation. The experimental result shows that ESCs can aggregate with each other close to the bottom of the liquid marble and form EBs, while remaining suspended in the culture media. The differentiation of the suspending EBs into contractile cardiomyocytes has been demonstrated inside the transparent liquid marbles, which enable the in situ microscopic observation. It was also found, through comparison, that ESCs in a bare sessile drop placed on a superhydrophobic substrate tend to anchor onto the substrate and then differentiate following the normal way of cell spreading, i.e., withdrawal from the cell cycle, fusion with nascent myotubes, and final differentiation into cardiomyocytes. In contrast, liquid marble particle shells weaken the adhesion of spherical EBs to the substrate, encouraging them to differentiate in suspension into cardiomyocytes, without anchoring. The results of this study highlight the promising performance of liquid marbles as one-pot micro-bioreactors for EB formation and differentiation.
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